Custom Polyclonal Antibody Production
KareBayTM Biochem has extensive experience in generating high quality antisera to a wide variety of antigens (peptides, proteins and other molecules). Using a low dose immunization level technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against an immunogen in mice, rats, and rabbits.
Polyclonal Antibody Production Protocol:
|
Time (Days)
|
Phase
|
Description
|
|
1
|
Receive the antigen provided by customer | Start the preparation of polyclonal antibody |
|
2
|
Detect the antigen by SDS-PAGE | Detect the protein molecular weight, purity and concentration |
|
4
|
Select two New Zealand rabbits | Coated ELISA plate by immunogen and select two New Zealand rabbits of low serum background |
|
6
|
Primary immune | Antigen+Freund’s complete adjuvant (FCA) |
|
22
|
Secondary immune | Antigen+Freund’s incomplete adjuvant (FIA) |
|
30
|
The third immune | Antigen+FIA |
|
38
|
Collect blood for ELISA detection | Antiserum titer detection by ELISA method If titer >10,000, collect blood for antibody production If titer<10,000, continue the fourth immune |
|
45
|
The fourth immune | Antigen+FIA |
|
52
|
Collect blood for ELISA detection | Antiserum titer detection by ELISA method If titer >10,000, collect blood for antibody production If titer<10,000, continue the fifth immune and contact customer |
|
55
|
Collect blood for antiserum preparation | 50-80mL antiserum could be obtained from the two rabbits |
|
60
|
Delivery | Pre-immune serum 1-3ml Antiserum 60-80ml ELISA detection report Experiment reports |
Services Specifications:
|
Cat.No.
|
Services
|
Description
|
Timeline
|
Price
|
|
KB035
KB188
|
Peptide Synthesis | Purity>85% Purity>95% |
2-3wks
|
Quote
|
|
KB036
|
Conjugation | KLH/BSA/OVA |
1wks
|
Quote
|
|
KB037
|
Antiserum Production | Immunize two rabbits Immunize five BALB/c mice Immunize three rats or enriettiis |
8-10wks
|
Quote
|
|
KB038
|
Antiserum Production | Immunize one rabbit |
8-10wks
|
Quote
|
|
KB041
|
From peptide to Antiserum | The final product is antiserum |
10-13wks
|
Quote
|
|
KB042
|
From peptide to Protein A purification | The final product is protein A purified antibody |
11-14wks
|
Quote
|
|
KB043
|
From peptide to antigen affinity purification | The final product is antigen affinity purified antibody |
12-15wks
|
Quote
|
|
KB044
|
From Protein to Antiserum | The final product is antiserum |
8-10wks
|
Quote
|
|
KB045
|
From protein to protein A purification | The final product is protein A purified antibody |
9-11wks
|
Quote
|
|
KB046
|
From Protein to Antigen Affinity Purification | The final product is antigen affinity purified antibody |
10-12wks
|
Quote
|
|
KB208
|
From peptide to Antiserum (Including WB detection) | Antiserum WB detection |
11-14wks
|
Quote
|
|
KB209
|
From peptide to Protein A purification (Including WB detection) | Antiserum and antibody WB detection |
13-16wks
|
Quote
|
|
KB210
|
From peptide to Antigen Affinity Purification (Including WB detection) | Antiserum and antibody WB detection |
14-17wks
|
Quote
|
|
KB211
|
From Protein to Antiserum (Including WB detection) | Antiserum WB detection |
9-11wks
|
Quote
|
|
KB212
|
From Protein to Protein A purification (Including WB detection) | Antiserum and antibody WB detection |
11-13wks
|
Quote
|
|
KB213
|
From Protein to Antigen Affinity Purification (Including WB detection) | Antiserum and antibody WB detection |
12-14wks
|
Quote
|
|
KB047
|
Phosphorylated Antibody Production | A phosphorylated peptide and a non-phosphorylated peptide |
12-15wks
|
Quote
|
|
KB197
|
Two Peptides to Antiserum Production (WB Certification, including WB detection) | The final product is WB positive antiserum |
11-14wks
|
Quote
|
|
KB187
|
Two peptides to Antigen Affinity purification (WB certification, including WB detection) | The final product is WB positive antiserum |
14-17wks
|
Quote
|
|
KB198
|
Three peptides to Antiserum Production (WB certification, including WB detection) | The final product is WB positive antiserum |
11-14wks
|
Quote
|
|
KB199
|
Three peptide to antigen Affinity purification (WB certification, including WB detection) | The final product is WB positive antiserum |
14-17wks
|
Quote
|
How to get started Please contact us with your custom polyclonal antibody production requests at service@karebaybio.com and we will reply with a detailed quote as soon as possible. This process usually takes between 24 and 48 hours and the quote will include an estimated price as well as the time required to complete the project. All inquiries and subsequent projects are handled in strict confidence and will be backed by a confidentiality agreement if desired.
