Custom Protein Expression Services

KareBay™ Biochem provides a comprehensive protein expression services and protein purification using both mammalian cells and bacteria systems. Generally, mammalian expression systems are suitable for glycosylated protein expression. Mammalian cells are widely used in the production of recombinant proteins and antibodies. Bacteria expression systems are preferred for non-glycosylated proteins because they are simpler, quicker and less expensive. Recombinant proteins are mainly used for biochemical and functional assays, antigen generation and a variety of other applications. We also offer a full service for protein purification. Our objective is to ensure efficient and high quality protein production for your R&D needs.

In addition, we perform a series of protein characterization and protein-based assays to meet your needs. It is worth mentioning that various enzyme activity-based assays allow high throughput drug screening to be performed. For example, we can provide you with kinase and protease activity assay services, as well as related biochemicals such as inhibitors, activators and substrates.

Once your order is confirmed, Karebay Biochem will initiate your protein expression and protein purification project using a plasmid obtained either from gene synthesis or from your own laboratory. The desired gene will be subcloned into an expression vector to express the targeted protein in bacteria. One liter of culture will be grown using our optimized protocol. Proteins can be purified using any common epitope tag of interest (FLAG, HIS, V5, c-myc, and HA).

Service Specifications:

Service	Description	Timeline	Price
Cloning into bacterial expression vector	Amplification of the gene of interest and subcloning it into a bacterial expression vector; Verification of the authenticity of the Subcloned gene by restriction enzyme digest and sequencing; Maxi-prep of the recombinant vector DNA.	2wks	Quote
Generation and identification of bacterial colonies; Expressing target proteins.	Transformation of recombinant constructs into high efficiency expression bacterial strain; Mini-induction to over-express the target protein; Test for the expression of the recombinant protein by western blot with anti-tag antibody (customer must provide appropriate antibodies) if desired.	2wks	Quote
Large-Scale Culture	One liters of bacterial culture will be induced with IPTG and harvested for protein purification.	1wk	Quote
Purification	Protein purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions.	1wk	Quote
Total Price per Protein			Quote

How to get started